Dig oligonucleotide 3 -end labeling kit 2nd generation manual

 · DNA sequences were determined with ABI Prism Big Dye Terminator cycle sequencing ready reaction kit and ABI DNA sequencer (PE Biosystems) in the Center for Gene Research of Yamaguchi University. Southern hybridizationThe oligonucleotide was labeled by ECL 3′-oligo labeling kit (Amersham Pharmacia Biotech).Cited by: Oligonucleotide and cRNA probes were used for nonradioactivein situ hybridizations carried out to identify the neural cell types expressing the glutamate transporter GLAST mRNA in the rat CNS. Additionally, the regional distribution of GLAST mRNA-expressing Cited by:  · Digoxigenin (DIG)-labeled riboprobes for fast MHC and desmin were generated in the sense and antisense directions in an in vitro transcription reaction using the DIG RNA labeling kit (Roche, Germany). Cryosections (12–14 μm thick) from adult tails were collected on SuperFrost charged glass slides (VWR, Chester, PA, USA).

(b) Two-day-old tomato seedlings were treated with a broad range of SNP concentration for 1 d. (c) Tomato seedlings were treated with H 2 O, 10 −2 µ m or µ m SNP for 1, 2 or 3 d. In another set of experiments (indicated as SNP µ m → SNP µ m), seedlings treated with µ m SNP for 1 d were shifted to fresh µ m SNP in. of DIG-High Prime and contents were briefly centrifuged. In order to increase DIG-labeled DNA yield, the sample 1. DIG High Prime DNA Labeling and Detection Starter was incubated overnight at 37°C in an MJ Mini Thermal Kit I (Roche Applied Science, Indianapolis, IN, Cat. Cycler (Bio-Rad, Hercules, CA). generation, validation, and application of the 12 strain-specific DNA probes have been described in detail elsewhere (). Purified DNA probes (n ⫽ 12) were spotted (1 ␮ l) onto a.

Ethidium bromide (EtBr) is a fluorescent dye widely used in molecular biology research. Early usage was as a veterinary trypanocide. 14 It is a mutagenic compound that intercalates double-stranded DNA and RNA. 1,10 The fluorescence of EtBr increases fold upon binding to double-stranded RNA, fold on binding double-stranded DNA (although. generation, validation, and application of the 12 strain-specific DNA probes have been described in detail elsewhere (13–15). Purified DNA probes (n ⫽ 12) were spotted (1 ␮ l) onto a. Services. We provide a wide variety of services related to many products, processes, and industries. Please select one of the categories below to learn more. Characterization. Contract Manufacturing. Custom Products. Product Services. Software Digital Platforms. Support.