Lambda zap ii manual

A lambda Insertion type cDNA cloning vector, Lambda ZAP, has been constructed. In E. coli a phagemid, pBluescript SK(−), contained within the vector, . Lambda ZAP II Predigested EcoR I/CIAP-Treated Vector Kit INSTRUCTION MANUAL Catalog # (Lambda ZAP II Predigested Vector Kit) and # (Lambda ZAP II Predigested Vector Kit with Gigapack III Gold Packaging Extract) . Lambda ZAP II vector can be screened with either DNA probes or antibody probes and allows in vivo rapid excision of the pBluescript phagemid, allowing your insert to be characterized in a plasmid system. The polylinker of the pBluescript phagemid, derived from .

Transient expression of heme-thiolate P Initial experiments assessed the level of enzyme activity from transiently expressed Md-C4H1 and the effect of Ad-P reductase 10 days after Agrobacterium infiltration. The extent of conversion from cinnamic acid to p-coumaric acid, measured by HPLC, was similar for both the MdC4H1 and the MdC4H1 + Ad-P reductase, and significantly higher than. ZAP-cDNA® Synthesis Kit, and ZAP-cDNA® Gigapack® III Gold Cloning Kit INSTRUCTION MANUAL Uni-ZAP® XR vector (i.e., Lambda ZAP® II vector digested with EcoR I and Xho I, CIAP treated)b 12 µg — 12 µg pBR test insert digested with Sal I (compatible with. host strain XL1Blue MRF´ is supplied with the Lambda - ZAP II vector kit. 2. Because the Lambda ZAP II vector does not require a. supF. genotype, the amplified library grows very efficiently on the XL1-Blue MRF´ strain. In addition, use of the correct host strain is important when working with the Lambda ZAP II vector as the F´ episome.

The Lambda ZAP vector1, 2 system combines the high efficiency of lambda library construction and the convenience of a plasmid system (see Figure 1). The Lambda ZAP vector has six unique cloning sites that will accommodate DNA inserts from Lambda ZAP vector can be screened with either DNA probes or antibody. Lambda ZAP-CMV XR Library Construction Kit INSTRUCTION MANUAL Catalog # BN # Revision A For In Vitro Use Only A lambda Insertion type cDNA cloning vector, Lambda ZAP, has been constructed. In E. coli a phagemid, pBluescript SK(−), contained within the vector, can be excised by 11 or M13 helper phage.